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Fig. 3 | BMC Cancer

Fig. 3

From: Resveratrol induces autophagy-dependent apoptosis in HL-60 cells

Fig. 3

RSV induces HL-60 cell apoptosis through both the death receptor pathway and mitochondrial pathway. a HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, then western blot analysis was used for analyzing the expression of proteins related to the intrinsic pathway of apoptosis by probing for Bax, Bcl-2, p-Bad and Bad. β-Actin was used as a loading control. b HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times and western blot analysis was used for analyzing the expression of extrinsic apoptosis pathway-related proteins by probing for Fas, Fas-L, Cleaved caspase-8, and Bid. β-Actin used as a loading control. c HL-60 cells were treated with 0–100 μM RSV for 24 h or 50 μM RSV for 0~ 48 h, then the mean fluorescence intensity of Rho123 was analyzed. d HL-60 cells were treated with different concentrations of RSV alone or combined with Z-DEVD-FMK (10 μM) for 24 h, then cell proliferation was analyzed by MTT assay. Data are presented as mean ± S.D. of three independent experiments in duplicates. *p < 0.05, vs. control

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