Fig. 2

RSV induces HL-60 cell apoptosis in a dose- and time-dependent manner. a HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, and western blot analysis was used for analyzing the expression of cleaved caspase-3. β-Actin was used as an internal control for equal amounts of protein applied. b HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, then caspase-3 enzyme activity was analyzed using a commercial kit. c HL-60 cells were treated with different concentrations of RSV for 24 h, or treated with 50 μM RSV for different times, then cell apoptosis was analyzed by flow cytometry. The histogram represents quantification analysis based on three independent experiments. Columns indicate mean ± SD of three experiments, *p < 0.05 vs. respective control cells