Fig. 2
From: The PDGFRβ/ERK1/2 pathway regulates CDCP1 expression in triple-negative breast cancer
PDGFRβ regulates CDCP1 expression in TNBC cells. a WB analysis of PDGFRβ and CDCP1 in MDA-MB-231 cells transfected with 100 nM PDGFRβ siRNA or the appropriate negative control. Cells were harvested at 48 h post-transfection. b WB analysis of CDCP1 and phosphoERK1/2 (T202/Y204) in MDA-MB-231 cells transfected with 100 nM PDGFRβ siRNA or the appropriate negative control and with or without 5% WHF in culture medium for 24 h. Dottes lines demarcate juxtaposed images originating from separate lines of the same western blot. The fold-change increase in CDCP1, calculated by densitometry, was 2.3 and 1.6, respectively. Monoclonal anti-actin was used as the total protein loading control