Fig. 1
From: The PDGFRβ/ERK1/2 pathway regulates CDCP1 expression in triple-negative breast cancer
PDGF-BB stimulation upregulates CDCP1 in TNBC cells. a CDCP1 in MDA-MB-231 cells treated with various growth factors, cytokines, and chemokines for 48 h was analyzed by FACS and reported as percentage upregulation with respect to the maximum increase induced by WHFs (100%). Representative experiment. b WB analysis of CDCP1, phospho-CDCP1 (Y734), PDGFRβ, and phospho-PDGFRβ (Y751) in MDA-MB-231 cells treated with or without PDGF-BB 20 ng/ml for 48 h. The fold-change increase in phospho-CDCP1 and CDCP1, calculated by densitometry, was 1.6 and 1.9, respectively. c WB analysis of CDCP1 in MDA-MB-231 cells treated with or without PDGF-BB 20 ng/ml and/or cycloheximide (1 μM) for 24 h. Monoclonal anti-actin was used as the total protein loading control