Fig. 7
WIN-55 inhibits the proliferation of RCC cells into 3D spheres. Scheme of experiment design (a) (d) and (g). b Representative images of spheres formed by 786-O and ACHN cells without/with WIN-55 (0, 10 μM). RCC cells were not able to form spheres (day 6–7) when WIN-55 was added at the beginning (day 0) of the assay. c Quantitative analysis of RCC spheres from 786-O and ACHN cell lines treated with WIN-55 (0, 10 μM). d Representative images and quantitative analysis of spheres formed by RCC cells when WIN-55 was added at day 2 for 786-O cells (e) and day 3 for ACHN cells (f). The growth of the spheres was observed at day 6 (786-O) and day 7 (ACHN) for quantitative analysis. g Representative images and quantitative analysis of sphere formation when WIN-55 was added at day 6 (786-O) (h) and day 7 (ACHN) (i). The growth of the spheres was observed at day 11 (786-O) and day 12 (ACHN) for quantitative analysis. j Quantitative analysis of RCC spheres treated with WIN-55 (0, 10 μM) at day 11 (786-O) and day 12 (ACHN). D: day; scale bar 200 μm; * p < 0.05