Fig. 3
Flow cytometric and western immunoblot analysis of the CB1 and CB2 receptor proteins in RCC cells. Graphs showing the representative histograms of CB2-positive a and CB1-positive b cells from different RCC cell lines. In each of the RCC cell lines, the CB2 protein expression was higher than that of the CB1 protein. Gray-filled histogram, unstained cells; black line histogram, stained cells. c Quantitative data indicating the protein expression levels of the CB1 and CB2 receptors in RCC cells. d Western immunoblot of the CB1 and CB2 proteins in RCC cell lines. Healthy kidney ASE-5063 (ASE) cells were used as the positive control for the CB1 and CB2 proteins. The GAPDH protein was used as an internal control. Forty micrograms of total protein was loaded onto the gels in each case. In each lane, there were two bands of proteins, the top band for CB1 or CB2 and the lower band for GAPDH. e Quantitative analysis of the western blot