Fig. 4

ANGPTL4 affects osteoblastic differentiation of osteosarcoma cells (a, b) ANGPTL4 over-expressing OSA cells showed increased ALP production but (c, d) reduced mineralization as revealed by Alizarin Red staining. (e, f) Low concentrations (25 ng/ml) of the cleaved forms of ANGPTL4 (cANGPTL4 and nANGPTL4) promoted mineralization of OSA cells. *p < 0.05