Fig. 1

Tiam1 could interacted with SETDB1directly by GST- pull down and Cross-linking assays. A Schematic representation of the domain structure of Tiam1. PCER; C685;C751 and C1199 were four truncations constructed containing different domains. B Interaction sites was verified by GST-pull down and westernblot assays. Proteins pulled down by agarose beads with GST tag further were detected by westernblot, (a) purified Tiam1-PCER protein labelled with GST was co-incubated with purified SETDB1 and detected by westernblot after elution, as shown in figure.a, Tiam1-PCER could be detected but not STEDB1, indicating the Tiam1-PCER fragment has no binding sties with SETDB1 (b,c,d) purified Tiam1-C685 protein was obtained as indicated, as shown in above figures, Tiam1-C685, C751 and C1199 could be detected as well as STEDB1, indicating the these fragments have binding sties with SETDB1. C Cross-linking assay confirmed Tiam1could interacted with SETDB1