Fig. 7

Sanguinarine induces apoptosis in vivo through disassociation of STRAP and MELK. a The expression of MELK, p-MELK, STRAP and p-STRAP in tumor tissue was detected by western blotting analysis. b Representative images of immunohistochemical staining of Bax, MELK p-MELK, STRAP and p-STRAP in tumor tissues (200×) (Scale bar is 50 μm). c Primary antibodies of MELK and STRAP were added into the whole lysates from tumor tissue respectively. Co-IP MELK and STRAP was tested by western blotting. d Tumors from four groups were dissected, fixed and embedded using O.C.T gum. Interaction with STRAP and MELK was tested by double immune-fluorescence staining for MELK (green) and STRAP (red) in colon cancer tissues. Photoes were taken by inverted fluorescence microscope (400×) (Scale bar is 50 μm)