Fig. 6

Sanguinarine induces Bax-dependent disassociation between STRAP and MELK. a HCT116 WT and HCT116 Bax^−/− cells were treated with sanguinarine for 12 h. RNA was extracted and qRT-PCR were performed to analyze STRAP mRNA relative level. b Protein expression of MELK, STRAP, p-MELK and p-STRAP was detected by western blotting. c and d The interaction between MELK and STRAP was analyzed by Co-IP. The lysate was subjected to immunoprecipitation with MELK (c) and STRAP (d) antibody respectively. Interaction of MELK and STRAP was evaluated by western blotting. e SW480, HCT116 WT and HCT116 Bax^−/− cells were treated respectivly with sanguinarine for 12 h and fixed by 4% paraformaldehyde. Cells were incubated with antibodies respectively and then probed secondary antibodies conjugated with FITC. Immunofluorescence imagines were observed under confocal microscopy (1000×) (Scale bar is 10 μm). Data presented are showed as means ± SD from three independent experiments. ^# p < 0.01 indicates significant difference