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Fig. 3 | BMC Cancer

Fig. 3

From: Sanguinarine triggers intrinsic apoptosis to suppress colorectal cancer growth through disassociation between STRAP and MELK

Fig. 3

Sanguinarine-induced apoptosis of CRC cells is an intrinsic apoptosis. a The expression of proteins from SW480 cells treated with 2.4 μM sanguinarine of Bcl-2 and Bax were determined by Western blotting. b Change of cytochrome c in mitochondrial and cytoplasmic fractions was analyzed by western blotting. COXIV and β-Actin were used to confirm equal protein loading respectively. c Cells were treated with the indicated concentrations of sanguinarine. ROS levels were evaluated by flow cytometry. d Cells were treated with or without 2.4 μM sanguinarine for 12 h after pretreatment with or without 10 mM NAC, a ROS scavenger, for 1 h. ROS levels were evaluated by flow cytometry. e Mitochondria membrane potential (ΔΨm) of SW480 cells was evaluated by JC-1 ratio of red and green fluorescence after sanguinarine treatment for 48 h. f Whole cell lysates were subject to western-blotting analysis using an antibody against caspase-3, caspase-9, cleaved caspase-9 and PARP antibodies. g Cell viability was measured by MTT assay (n = 5). h Expression of the apoptosis proteins from SW480 cells treated with or without 2.4 μM sanguinarine after pretreatment with or without 10 mM NAC for 1 h. Data presented are showed as means ± SD from three independent experiments. * p < 0.05 and # p < 0.01 indicate significant difference

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