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Fig. 2 | BMC Cancer

Fig. 2

From: Sanguinarine triggers intrinsic apoptosis to suppress colorectal cancer growth through disassociation between STRAP and MELK

Fig. 2

Sanguinarine-induced apoptosis in CRC cells. a SW480 and HCT116 cells were treated with the indicated concentrations of sanguinarine for 24 h and 48 h. Cell viability was measured by MTT assay (n = 5). b Cells were stained by DNA-specific Hoechst 33,342 dye. Hoechst positive cells were analyzed quantitatively by calculating the percentage of the number of cells with condensed chromatin (n = 3). c Cells were stained with PE-Annexin V and 7-AAD and quantitatively analyzed by flow cytometry (n = 3). d Cells were pretreated with or without pan-caspase inhibitor, z-VAD-fmk (50 μM) respectively, for 1 h before explosure to sanguinarine for 48 h. Apoptosis cells were evaluated as described in (c) (n = 3). Data presented are showed as means ± SD from three independent experiments.* p < 0.05 and # p < 0.01 indicate significant difference

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