Fig. 8

Fluorescence intensity analysis of ceramide and DR5 after GT3 treatment in MIA PaCa-2 cells. a MIA PaCa-2 cells were treated with dissolution vehicle (A, top panels ) or 40μM GT3 (A, bottom panels), fixed, and processed for immunofluorescence. Single confocal sections shown in the x-y plane, show localization of DR5 (green channel), ceramide (red channel), and nuclei using DAPI (blue channel). b The fluorescence intensities along the white lines indicated in the merged pictures were quantified using the line scan application of Leica software. Data obtained from line scans drawn through the cell were plotted in a graph and expressed as mean ±SE, n= 50 cells, from three independent experiments.*p < 0.05