Fig. 4

TLR4 signaling is involved in NF-κB-mediated NEAT1 expression activation (a-b) A549 and H1299 cells were co-treated with LPS (20 ng/ml) and CLI-095 (50 nmol/L) for 4 h; the protein levels of TLR4, MyD88 and p-p65 were determined using Immunoblotting assays. c The mRNA expression of NEAT1 was determined using real-time PCR assays. The data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to control group; #P < 0.05, ##P < 0.01, LPS group compared to LPS + CLI-095 group