Fig. 4

mTOR signaling pathway was activated in HCC; a Immunohistochemistry of p-AKT (Ser473), p-mTOR (Ser2448), and p-S6 K1 (Thr389) in HCC clinic samples, the magnification of images was 400×; b Western blotting of AKT, p-AKT (Ser473), mTOR, p-mTOR (Ser2448), S6 K1 and p-S6 K1 (Thr389) in hepatocellular carcinoma cell lines (Bel-7402, QGY-7703, MHCC97L and MHCC97H). Control was a piercing liver tissue which was diagnose as hepatitis without tumor. The expression levels were normalized to GAPDH. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean ± SD. P-values were calculated using Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001