Fig. 2
From: Pan-RAF and MEK vertical inhibition enhances therapeutic response in non-V600 BRAF mutant cells
Signaling in sorafenib and/or selumetinib treated cells. Cells were treated with 1 μM of sorafenib and 50 nM of selumetinib as indicated for 4 h. Phosphorylation inhibition of Erk1/2 is enhanced by combination treatment. Single selumetinib treatment could increase Akt activation in WM3629 and WM3670 cell lines. Total expression of CRAF could be induced in non-V600 BRAF mutant cells but not in V600E BRAF mutant A375 cell. β-tubulin served as loading control. Blots are representative images from three independent experiments