Fig. 7
From: The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotype
Overexpressed Nup88 interacts with the N-terminal region of vimentin. Schematic of vimentin constructs used in the GFP-coprecipitation assay (a). Nup88 overexpressing cells that transiently co-overexpress either full-length vimentin (Vim-FLAG) or an N-terminal truncated vimentin (Δ1-96-FLAG) were subjected to the GFP-coprecipitation assay (b). Cell lysates containing Nup88-FLAG with either control GFP or 1-96-GFP were subjected to the GFP-coprecipitation assay (c). Interaction between the Nup88 truncation and the vimentin N-terminal region (d). Inputs are 6.0% for FLAG constructs and 4.0% for Nup88-GFP (b), 0.7% for Nup88-FLAG and 1.3% for GFP constructs (c), and 2.0% for both Nup88(1-541)-FLAG and GFP constructs (D), respectively. Arrows indicate Nup88-GFP (b) and 1-96-GFP (c and d), respectively