Fig. 3

Representative results of bisulfite genome sequencing for methylation analysis. a The bisulfite genome sequencing (BGS) analysis was performed on the α-internexin genomic region (+ 683~ + 834) relative to the transcription start site (TSS). DNA sequence of the region for BGS and methylation level of CpG sites are shown. b1 Pancreas neuroendocrine tumor, G1, α-internexin positive expression, 3 CpG sites were unmethylated (red arrow). b2 Rectum neuroendocrine carcinoma, G3, loss expression of α-internexin, 3 CpG sites were methylated (blue arrow)