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Fig. 5 | BMC Cancer

Fig. 5

From: Inhibition of glutamate oxaloacetate transaminase 1 in cancer cell lines results in altered metabolism with increased dependency of glucose

Fig. 5

Cell death and morphology in GOT1-null 143B and siRNA knock-down A549 cells upon glucose deprivation. a Cells grown in 1 g/L glucose, 0 g/L glucose or treated with 2 mM H2O2.for 24 h Arrows in black indicate ischemic-like cell death in GOT1-null 143B cells. Arrows in white indicate apoptotic cell death. Bars represent 25 μm. b A549 non-template control (NTC) and GOT1-siRNA A549 cells grown in 1 g/L glucose or 0 g/L glucose for 24 h. Arrows in black indicate ischemic-like cell death. Bars represent 25 μm. c Prevention of ischemic-like cell morphological changes by OAA and PEP in medium without glucose. Cells grown in 4.5 g/L glucose for 4 days, and then the old medium was replaced with glucose-free-medium containing 10 Mm Asp, 5 mM OAA or 2.5 mM PEP. The cell morphological changes 5 min after replacing nutrient-depleted-medium. d Prevention of ischemic-like cell morphological changes by OAA and PEP in medium with 4.5 g/L glucose. Cells grown in 4.5 g/L glucose for 4 days, and then the old medium was replaced with 4.5 g/L glucose-medium containing 10 Mm Asp, 5 mM OAA or 2.5 mM PEP. The cell morphological changes 5 min after replacing nutrient-depleted-medium. Bars indicate 25 μm

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