Fig. 1

Characterization of GOT1-null cells. a A single base pair CG deletion in exon 1 of GOT1 introduced via CRISPR/Cas9 in 143B cells. b Western-blot of GOT1. 1: wild type 143B; 2: mutated GOT1. c Expression levels of wild type and GOT1-null mRNA. d Growth curves of wild type and GOT1-null 143B cells. e Colony formation of GOT1-null 143B cells in soft agar. f Decreased migration of GOT1-null 143B cells. Bars indicate 50 μm. g AOA treatment in wild type 143B. h AOA treatment in wild type A549 cells. All the experiments have been repeated 3 times, and data are represented as mean ± s.d. Unpaired student’s t-test was performed for d, e. One-way ANOVA was performed for g and h. *** p < 0.001, ** p < 0.01, * p < 0.05; NS: not significant