Fig. 7

Analysis of cell viability and chemosensitivity after ROCKi treatment. a and b Measurement of cell viability with resazurin in NCH644 (a) and NCH421k (b) after Y-27632 treatment for 72 h. There is no significant decrease in cell viability after application of Y-27632 even at concentrations up to 50 μM (n = 3, one-way ANOVA followed by Dunnett’s multiple comparisons test, p > 0.05). Shown are means + SEM. (c) and (d) Fit of dose response curves to determine IC50 values with variable slope against the log transformed temozolomide concentration for NCH644 (c) and NCH421k (d). e Quantification of dose response curves as shown in (c) and (d). Temozolomide chemosensitivity changes depend on cellular background. While NCH644 cells are sensitized, NCH421k cells become more resistant by Y-27632 treatment (n = 3, one-way ANOVA followed by Dunnett’s multiple comparisons test, significant changes are indicated). Shown are means + SD. f Western blot after PhosTag gel electrophoresis probed with α-cofilin antibody. Inhibitor treatment with Y-27632 and LIMKi3 strongly reduces the levels of p-cofilin. Treatment with fasudil is less effective, especially in stem-like cells