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Fig. 4 | BMC Cancer

Fig. 4

From: Chronophin regulates active vitamin B6 levels and transcriptomic features of glioblastoma cell lines cultured under non-adherent, serum-free conditions

Fig. 4

Changes in cellular phenotypes after CIN knockdown. a and b Active vitamin B6 levels are significantly increased after CIN knockdown in NCH644 a and NCH421k b (n = 3, one-way ANOVA followed by Dunnett’s multiple comparisons test, p < 0.05 and 0.001, respectively). Shown are means + SD. c and d Colony formation assay in a collagen matrix. Colony numbers are significantly reduced after CIN knockdown in NCH644 (n = 3, generalized linear model (poisson family with link function log), p < 0.01 for both CIN shRNAs) but not in NCH421k (d). Shown are means +SD. e and f Colony formation assay in a collagen matrix. Colony numbers are significantly increased after treatment with 10 μM Y-27632 in NCH644 e (n = 3, generalized linear model (poisson family with link function log), p < 0.01 for 10 μM Y-27632, p > 0.05 for 1 μM Y-27632) but not in NCH421k (f). Shown are means +SD

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