Fig. 5
From: Development of a computational promoter with highly efficient expression in tumors
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, HEK293 and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)