Fig. 6

Confocal microscopy images of HER2-positive SKBR-3 breast cancer cells treated with Type B exosomes. a–c No T-DM1 is detected on the SKBR-3 cells treated with exosomes harvested from fresh cell culture medium of PBS-treated SKBR-3 cells (negative control). d–f Intense membranous staining is present when the cells were treated with T-DM1 (positive control). g–i T-DM1 is present on cells treated with exosomes obtained from fresh cell culture medium of T-DM1-treated cells. The images were taken with identical microscope settings