Table 1 List of commercial reagents used in this study
From: Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue
Name | Company (Country) | Catalog Number | Comments |
|---|---|---|---|
Lab vision PT Module | Thermo scientific (USA) | ||
Trilogy Pretreatment Solution | Cell Marque (USA) | 920P | Diluted to 1× with ddH2O |
Ethanol | AGA (Portugal) | 4.006.16.00.00 | Used in 70% and 96% solution and pure. |
Peroxidase block solution | Atom Scientific (United Kingdom) | GPC8054-E | |
TBS IHC wash buffer with Tween 20 | Cell Marque | 935B-09 | Diluted to 1× with ddH2O |
Diamond antibody diluents | Cell Marque | 938B-09 | |
Hi-Def Detection HRP Polymer System | Cell Marque | 954D-30 | A polymer containing anti-rat Ig Fc conjugated with HRP |
DAB Chromogen/Substrate Bulk Pack (High Contrast) | ScyTek Laboratories (USA) | ACV500 | A pack of chromogen concentrate, the 3,3′-Diaminobenzidine tetrahydrochloride (DAB) and DAB substrate |
Xylene | Klinipath Netherland (The Netherland) | 4055.9005 | |
Mayer’s Hematoxylin | Bio-Optica (Italy) | 05–06002/L | |
Quick-D Mounting Medium | Klinipath Nederland | 7281 | |
Coverslips | Thermo Scientific | 4951PLUS4 | |
Humidity chamber | Bio-Optica | ||
E-Ig chimera | R&D Systems (USA) | 575-ES-100 | Diluted to 1:300 in Diamond: Antibody Diluent |
Rat anti-mouse CD62E | BD Biosystems (USA) | 550,290 | Diluted to 1:250 in Diamond: Antibody Diluent |
Anti-sLeX/A (HECA-452) | Biolegend (USA) | 321,302 | Diluted to 1:50 in Diamond: Antibody Diluent |