Fig. 2
From: Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue
Immunohistochemistry staining of colon adenocarcinoma tissue with E-Ig chimera. Brown color indicates positive reactivity and shows expression of E-selectin ligands in a serial section of the same tissue with 40× magnification (a); 400× magnification (b), 100× magnification (c) and 600× magnification (d). b is an increased magnification of the boxed area shown in a, demonstrating the high reactivity with goblet cells (indicated by black arrows) of the crypts and in particular in their apical pole. In d it is highlighted the positive reactivity within the lumens of the crypts and in the cellular cytoplasm (indicated by black arrow). In a and c the asterisks show the lamina propria with no staining, except for nests of neoplastic cells indicated by the blue arrows