Table 2 Results obtained from samples of the round robin test. Reference values were obtained from the trial coordinators by a multiplex assay analyzed on a capillary sequencer and reported to our laboratory. Afterwards, the samples were re-analyzed on the QiaXcel Advanced system. All samples could be fully confirmed by analyzing the peak patterns
From: Microsatellite instability testing in colorectal cancer using the QiaXcel advanced platform
Round Robin Sample | BAT25 | BAT26 | D5s346 | D2S123 | D17s250 | MS-status | |
|---|---|---|---|---|---|---|---|
1 | expected | wt | wt | wt | wt | wt | MSS/MSI-L |
our result | wt | wt | wt | wt | wt | MSS | |
2 | expected | MSI | MSI | MSI | MSI | MSI | MSI-H |
our result | MSI | MSI | MSI | MSI | MSI | MSI-H | |
3 | expected | wt | wt | wt | wt | wt | MSS/MSI-L |
our result | wt | wt | wt | wt | wt | MSS | |
4 | expected | MSI | MSI | MSI | MSI | MSI | MSI-H |
our result | MSI | MSI | MSI | MSI | MSI | MSI-H | |
5 | expected | wt | wt | wt | wt | wt | MSS/MSI-L |
our result | wt | wt | wt | wt | wt | MSS | |
6 | expected | wt | wt | wt | wt | wt | MSS/MSI-L |
our result | wt | wt | wt | wt | wt | MSS | |
7 | expected | MSI | MSI | MSI | MSI | MSI | MSI-H |
our result | MSI | MSI | MSI | MSI | MSI | MSI-H | |
8 | expected | MSI | MSI | MSI | MSI | MSI | MSI-H |
our result | MSI | MSI | MSI | MSI | MSI | MSI-H | |