Table 1 Forward and reverse primer used for the detection of the respective microsatellites
From: Microsatellite instability testing in colorectal cancer using the QiaXcel advanced platform
Microsatellite | Forward-primer | Reverse-primer |
|---|---|---|
BAT 25 | TCGGCTCCAAGAATGTAAGT | TCTGCATTTTAACTATGGCTC |
BAT 26 | TGACTACTTTTGACTTCAGCC | AACCATTCAACATTTTTAACCC |
BAT 40 | GTAGAGCAAGACCACCTTG | ATTAACTTCCTACACCACAAC |
D2s123 | AAACAGGATGCCTGCCTTTA | GGACTTTCCACCTATGGGAC |
D10s197 | GTGATACTGTCCTCAGGTCTCC | ACCACTGCACTTCAGGTGAC |
D13s153 | AGCATTGTTTCATGTTGGTG | CAGCAGTGAAGGTCTAAGCC |
D17s250 | GGAAGAATCAAATAGACAAT | GCTGGCCATATATATATTTAAACC |
D18s58 | GCAGGAAATCGCAGGAACTT | GCTCCCGGCTGGTTTT |
D5s346 | ACTCACTCTAGTGATAAATCG | AGCAGATAAGACAGTATTACTAGTT |
MycI | CCTTTTAAGCTGCAACAATTTC | TGGCGAGACTCCATCAAAG |