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Fig. 4 | BMC Cancer

Fig. 4

From: NKX2.5 is expressed in papillary thyroid carcinomas and regulates differentiation in thyroid cells

Fig. 4

Effect of NKX2.5 on the expression and activity of DuOx: PCCL3 cells were transfected with empty vector (pcDNA3.1) or with the plasmid encoding for NKX2.5 (pNKX2.5) and we have evaluated mRNA levels of the following genes: (a) DuOx1, (b) DuOxA1, (c) DuOx2 and (d) DuOxA2, as well as (e) extracellular H2O2 generation (for RT-PCR, n = 7 per condition and for DuOx activity, n = 6 per condition; ** p < 0.01 vs. pcDNA3.1); (f) Wild type NKX2.5 (Nkx2.5 wt) and mutant NKX2.5 (Ile183 to Pro mutation - Nkx2.5 mut) were transfected in HEK293T cells and the activity of pDuOx1 (DuOx1 promoter) was evaluated. Empty vector pcDNA3.1 was transfected as control. Renilla was co-transfected in HEK293T cells to normalize luciferase activity. All transfections were done in triplicate at least twice (n = 6 per condition). *** p < 0.001 vs. pcDNA3.1. Data are expressed as relative to control (empty vector - pcDNA3.1). DuOx1 – dual oxidase 1; DuOx2 – dual oxidase 2; DuOxA1 - dual oxidase maturation factor 1; DuOxA2 - dual oxidase maturation factor 2

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