Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: NKX2.5 is expressed in papillary thyroid carcinomas and regulates differentiation in thyroid cells

Fig. 2

NKX2.5-overexpressing PCCL3 cell viability. The cells were transfected with empty vector (pcDNA3.1) or with the plasmid encoding for NKX2.5 (pNKX2.5) and overexpression was confirmed by (a) mRNA (n = 7 per condition; *** p < 0.001 vs. pcDNA3.1) and (b) protein levels (n = 2). (c) Cell viability, evaluated by MTT assay, performed 0, 24, 48 and 72 h after transfection (n = 8 per condition). GAPDH was used as loading control for Western blot analysis and RPL4 for RT-PCR

Back to article page