Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress

Table 5 RT qPCR shows negative fold change of gene expression in PAX6 KO cells compared to WT. ∆∆Ct method was used for analysis

Gene abbreviation	Protein name	Fold change # ± SD
MMP2	Matrix metalloproteinase 2	−5.0 ± 1.50
CAV-1	Caveolin-1	−2.0 ± 0.24
CCND1/ cyclin D1	Cyclin D1	−2.4 ± 0.14
CDKN1B/ p27	Cyclin-dependent kinase inhibitor 1B (p27^Kip1)	−1.5
NFE2L2 / Nrf2	Nuclear factor (erythroid-derived2)-like 2 NFE2L2 or Nrf2	−3.7 ± 0.68

The relative amount of target gene normalized to the average expression of the two reference genes TFRC and GAPDH was determined using the ΔΔCt-method [71]. WT values were put as 1, and fold change according to this were calculated for the other cell types/treatments

ISSN: 1471-2407