Fig. 4

PAX6 KO cells migrate faster than WT and control cells. a WT U251 N cells, pooled PAX6 control cells and pooled PAX6 KO cells were seeded in Ibdi 2-well silicone inserts and left for 24 h. Cells were Mitomycin C treated for 2 h before inserts were removed, creating a cell free area (gap) at the start of the assay. Cells were micro-photographed at 0, 20, 48, and 72 h after well inserts were removed. The white lines demark migration edges. b The average migration distance was calculated after 20 h as described in methods. Students t-test showed that the difference in migration distance observed between pooled PAX6 KO cells and WT U251 N was significant (p < 0.002). The difference between pooled PAX6 Control cells and WT U251 N cells was not. Migration assays were repeated three times. Each assay showed the same result