Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: C-Cbl reverses HER2-mediated tamoxifen resistance in human breast cancer cells

Fig. 4

Knockdown of HER2 expression in BT474 cells suppresses the formation of the ER-c-Src-HER2 complex and partially restores tamoxifen sensitivity. a Western blot analysis of BT474 cells transfected with siRNA targeting HER2. b BT474 cells transfected with HER2 siRNA were subjected to immunoprecipitation and immunoblotting as indicated. c Western blot analysis of phosphorylated HER2, c-Src, and ER in BT474 cells knocked down for HER2 expression for 48 h and then treated with 17β-E2 (10 nmol/L) and/or tamoxifen (TAM) (1 μmol/L) for 4 h. d MTT assays in BT474 cells transfected with siRNA targeting HER2 for 24 h and then treated with 17β-E2 (10 nmol/L) and/or tamoxifen (TAM) (1 μmol/L) for another 48 h. e Colony formation assays of BT474 cells transfected with siRNA targeting HER2 for 24 h and treated with 17β-E2 (10 nmol/L) and/or tamoxifen (TAM) (1 μmol/L) for 14d

Back to article page