Table 1 All 5′ primers contain a SacI and all 3′ primers a BglII site for cloning into pGl3-P vector
Name BRE | 5′ primer (5′-3′) | 3′ primer (5′-3′) | size (bp) |
|---|---|---|---|
7–13 | GATGAGCTCATGCGAAAGTGAAGGTGGTCCCCAG | TGAAGATCTAGCAAGGAGGAACTGGAGATGTCA | 1278 |
9–13 | GAGCTCTCGAACCTCTCTTTCCTATCTC | AGATCTAGCAAGGAGGAACTGGAGATGTCA | 695 |
10–13 | GAGCTCTGGAGCTGAGCCTGGAGAGAAG | AGATCTAGCAAGGAGGAACTGGAGATGTCA | 530 |
12–13 | GAGCTCTAGGCCATCAGACAGCTCTGAG | AGATCTAGCAAGGAGGAACTGGAGATGTCA | 127 |
9 | GAGCTCTCGAACCTCTCTTTCCTATCTC | AGATCTCTTCTCTCCAGGCTCAGCTCCA | 189 |
9–10 | GAGCTCTCGAACCTCTCTTTCCTATCTC | AGATCTCCCTCATCTCTCCTTGCAGCT | 253 |
9–11 | GAGCTCTCGAACCTCTCTTTCCTATCTC | AGATCTAGGCCCTCAAATTCCTCAGAG | 348 |