Fig. 6

Intracellular localization of calretinin and septin 7 in MM cells a Septin 7 localization (red; a1) and CR (green; a2) in MSTO-211H cells treated with 1 mM Bt. Cortical regions (arrows) and cytoskeletal-like structures (filaments, bundles; arrowheads) are strongly stained for septin 7. a3 Nuclear DAPI staining. a4) Merged images a1–3). Scale bars: 10 μm. b Localization of septin 7 (red) and CR (green) at different stages of cytokinesis starting from early telophase b1 until close to cell separation b8] in MM cell lines. Yellow indicates colocalization of the 2 proteins. DAPI-stained nuclei are shown in blue. Scale bars in all images: 10 μm. 1) MSTO-211H-CR; 2) MSTO-211H treated with 1 mM Bt; 3) MSTO-211H-CR; 4) MSTO-211H treated with 1 mM Bt; 5) MSTO-211H-CR; 6) SPC111-CR; 7) ZL55-CR; 8) SPC212-CR. c Co-IP with lysates from MSTO-211H-CR and ZL55-CR cells. Proteins binding to CR were co-immunoprecipitated with a CR antibody. Membranes with PAGE-separated proteins were probed for CR and septin 7 (lane IP). Input samples containing all proteins (prior to immunoprecipitation) were probed for the same proteins. Paxillin was used as negative control