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Fig. 4 | BMC Cancer

Fig. 4

From: Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter

Fig. 4

Analysis of septin 7 and CR levels in different cell lines of mesothelial origin and after experimental manipulations. a Relative expression levels of CR and septin 7 in various cell lines of mesothelial origin. b Semi-quantification of CR and septin 7: brown dots represent immortalized mesothelial cells, red dots epithelioid MM, orange dots bi-phasic and yellow dots sarcomatoid MM cell lines. c Effect of Bt administration on CR and septin 7 levels in MSTO-211H (C1) and ZL5 cells (C2). d Extracts of MSTO-211H cells infected with LV-septin7 or LV-GFP; note the decrease in CR in cells overexpressing septin 7. e MSTO-211H cells treated with Bt (+) or not (−) and subjected to down-regulation of septin 7 by shSEPT7; shGFP served as a negative control. f Up-regulation of CR by LV-CALB2 in SPC212 and HT-29 cells; note the decrease in septin 7 levels. The complete Ponceau-stained membranes corresponding to the Western blots shown in a and d are shown in Additional file 1: Figure S3

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