Fig. 2

Sequence of the human CALB2 promoter region containing putative Bt-responsive elements BRE7–13 a The region of 1278 bp contains 7 putative BREs (cyan). Primer sequences to amplify the 1278-bp fragment are marked in yellow including restriction sites for SacI (Table 1) and BglII (red). The start site of translation is marked in red (bold). Primers used to amplify truncated versions are boxed in gray (details are shown in Table 1). b Sequence alignment of BRE5 - BRE13 with the BRE consensus sequence (15) shown at the bottom. Nucleotide sequences fully complying with the consensus (100% identity) are marked in blue, medium-conserved sequences are marked in green and nucleotides not conferring to the consensus sequence are marked in black; percentages of identity with the consensus sequence are given in the right lane. c, d Luc + reporter assay in MSTO-211H (upper) and ZL55 MM (lower) cells with CALB2 promoter fragments containing variable numbers of BREs. Luc + activities were normalized to the signal obtained with the control plasmid pGl3-P. As reference for the statistical analysis, BRE5–6 [15] was used. The number of independent experiments ranged from n = 4 (e.g. BRE10–13) to n = 11 (e.g. BRE7–13). Values represent mean ± S.D. *p < 0.05 vs. BRE5–6