Fig. 4

CXCL11 induced PD-L1 upregulation and the activation of STAT3 and Akt. a: SGC7901 cells were pretreated with or without STATTIC (5 μM) overnight followed by CXCL11 (100 ng/mL) stimulation for 5 min and 72 h, the phosphorylation of STAT3 and PD-L1 were analyzed by Western blot. b: SGC7901 cells were pretreated with or without LY294002 (25 μM) for 2 h followed by CXCL11 (100 ng/mL) stimulation for 5 min and 72 h, the phosphorylation of Akt and PD-L1 were analyzed by Western blot. c: SGC7901 cells were pretreated with or without AKT siRNA for 24 h, followed by CXCL11 (100 ng/mL) stimulation for 5 min and 72 h, the phosphorylation of Akt and PD-L1 were analyzed by Western blot. Normalized protein expression levels were calculated and analyzed. The gels were run under the same experimental conditions. The band intensities were calculated using the ImageJ 1.46r software. GAPDH was used as an internal control for the total protein measurement. The ratio of the target gene to GAPDH was used to conduct the statistical analysis. *P < 0.05 and **P < 0.01, as determined by Student’s t-test