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Fig. 2 | BMC Cancer

Fig. 2

From: CXCL9/10/11, a regulator of PD-L1 expression in gastric cancer

Fig. 2

CXCL9/10/11 upregulated PD-L1 expression in gastric cancer cells and tissues. a: CXCR3 and PD-L1 expression were analyzed by Western blotand IHC. Two representative cases from 92 clinical gastric cancer specimens were shown. The gene expression level was evaluated in three random visual fields. Original magnifications: 200×. b: SGC7901 and MKN74 cells were incubated with CXCL9/10/11 (100 ng/mL) for 72 h, the PD-L1 expression was analyzed by Western blot. c: SGC7901 cells were pretreated with or without AMG487 (1 μM) for 2 h followed by CXCL9/10/11 (100 ng/mL) stimulation for 72 h. Cell lysates were collected for Western blot analysis. Normalized protein expression levels were calculated and analyzed. The gels were run under the same experimental conditions. The band intensities were calculated using the ImageJ 1.46r software. GAPDH was used as an internal control for the total protein measurement. The ratio of the target gene to GAPDH was used to conduct the statistical analysis. *P < 0.05 and **P < 0.01, as determined by Student’s t-test

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