Fig. 7

TGF-β inhibitor Galunisertib inhibits mobility of gastric cancer cell lines. (a) The level of SERPINE1 in mock-, DMSO-, and 400 mM UK 383367-treated MKN45 was determined by RNA sequencing. (b) The expression level of TGF-β and LTBP-1 in AGS, MKN28, and MKN45 was determined by RNA sequencing. (c) Total RNA was purified from AGS, MKN28, and MKN45 was treated with 10 ng/ml recombinant TGF-β for 0, 2, 4, and 6 h. The level of SERPINE1 was determine by qRT-PCR. (d) MKN28 and AGS were treated with 400 mM Galunisertib. The mobility was assessed using wound healing assay as described in Fig. 5b