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Fig. 2 | BMC Cancer

Fig. 2

From: microRNA-451a regulates colorectal cancer proliferation in response to radiation

Fig. 2

Ectopic expression of miR-451a inhibits proliferation and clonogenic survival of HCT-116 cells. A) HCT-116 were transfected with a miR-451a mimic or a control mimic at the indicated doses. Proliferation was analyzed 48 h after radiation with the indicated doses. Bars depict mean ± s.e.m. of triplicate wells. * indicates P < 0.05 on ANOVA for interaction between radiation and miR treatments. b HCT-116 cells were transfected as in a and cell cycle analysis was performed via flow cytometry of propidium iodide stained cells at the indicated time points. c HCT-116 cells were transfected and irradiated as described in A and plated 24 h later. 12–14 days after plating, cells were fixed and stained with crystal violet and colonies were counted. Surviving fraction was calculated based on the colony numbers normalized to the plating efficiency. Mean of triplicate wells is plotted. * indicates P < 0.05 and ** indicates P < 0.01on a two-tailed Student’s T-test

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