Fig. 2
From: Small cell and non small cell lung cancer form metastasis on cellular 4D lung model
Non small cell lung cancer (NSCLC) cells on the cellular 4D lung model. Primary tumors were formed in a bronchocentric fashion on the cellular lung upon A549 (a–d), H1299 (e–h) and H460 (i–l) cell seeding though the trachea. Human mitochondrial IHC staining clearly shows the presence of a tumor formed by human lung cancer cells in a rat lung after 12 days of culture in a bioreactor. Low and high power H&E staining indicates the presence of a microscopic tumor with distinctive histology based on type of cell placed in the model. A549 cells formed a focal acinar pattern with enlarged nuclei and scattered atypical mitotic cells (c and d). H1299 cells formed a solid tumor and resemble poorly differentiated cells with scattered atypical mitosis (g and h). H460 cells formed a solid tumor with vague glandular formation (k and l)