Fig. 5

Profiling of apoptosis related proteins in CT26 and B16F10 cells after the combined treatment. ILz:rhTRAIL (100 ng/ml) and bortezomib (100 nM) were treated into CT26 (a) and B16F10 cells with or without pre-treatment with z-VAD-fmk: ILz:T, ILz:rhTRAIL; B, bortezomib; Z, z-VAD-fmk (b). After the indicated time periods, live and dead cells were harvested. Cell lysates were prepared using RIPA buffer and immunoblotting analysis was performed with appropriate antibodies, as described in ‘Materials and Methods’. Caspase-3 and -8 refer to cleaved caspases-3 and -8, respectively. Bax (40 kDa) refers to dimerized Bax. Actin was used as a loading control. *p < 0.05 by Student’s t-test