Table 2 The primers for real-time PCR and semi-quantitative RT-PCR used in this study
From: The oncogenic effects of HES1 on salivary adenoid cystic carcinoma cell growth and metastasis
Gene | Accession no. | Forward | Reverse |
|---|---|---|---|
ACTB | NM 001101 | CCTGGCACCCAGCACAAT | GGGCCGGACTCGTCATACT |
HES1 | NM 005524.3 | AGGCGGACATTCTGGAAATG | CGGTACTTCCCCAGCACACTT |
NOTCH1 | NM 017617.3 | GGAAGTTGAACGAGCATAGTCC | GCATGATGCCTACATTTCAAGA |
CCND1 | NM 053056.2 | CCCCGCACGATTTCATTGAACA | CATGGAGGGCGGATTGGAAATG |
Ki67 | NM 002417.4 | GCTCCCCACCTCAGAGAGTTTT | CTCTTAAGGGAGGGCTTGCAGA |
KRT14 | NM 000526.4 | GAGCCGCATTCTGAACGAGATG | ACTGCAGCTCAATCTCCAGGTT |
IGFBP7 | NM 001553.2 | GATGCTGGAGAATATGAGTGCC | CCATGACTACTTTTAACCATGCA |
PSCA | NM 005672.4 | CCAGGTGAGCAACGAGGACT | TAGTCCTGTGAGTCATCCACGC |
S100A2 | NM 005978.3 | ATAAATCCTCACCCTGGGAGCC | CCCTCTTGGCAGGAGTACTTGT |
C9ORF3 | NM 032823.5 | TCTGCGGAAGTGGTGACCC | AGGGTCCAGCTGTATGTCCATG |
MMP9 | NM 004994.2 | GAGGCGCTCATGTACCCTATGT | GGTGTGGTGGTGGTTGGAGG |