Fig. 4
From: ZBP-89 and Sp1 contribute to Bak expression in hepatocellular carcinoma cells
Sp1 siRNA interference decrease Bak expression. Figure showed a reduction effect on Bak expression when endogenous Sp1 was decreased by siRNA using Western blotting method (A and B, * p < 0.05 compared with the corresponding control). When pretreated with Sp1 expression vector, Sp1 siRNA could also decrease Bak expression level in the presence of MIHA (30 nM) in PLC/PRF/5 and HKCI-4 cells. In RT-PCR experiment of Bak mRNA level examination, data showed that Sp1 siRNA blocked Bak transcription, even in the presence of MMA treatment in MIHA, LO2, HKCI-4 and PLC/PRF/5 cells (C, * p < 0.05 compared with the corresponding control, 4-well repeated in triple experiments, data shown as Mean ± SE)