Fig. 1
From: Active DNA end processing in micronuclei of ovarian cancer cells
Genotoxicity-induced micronuclei in ovarian cancer cells. a: Induction of DSBs in OVCAR-8 cells after 1 Gy ionizing radiation. γH2AX and 53BP1 foci and nuclei morphology were monitored by indirect immunofluorescent staining (IF) at indicated time post-IR. Nuclei were indicated by DAPI staining. b: Dynamics of γH2AX IRIF in OVCAR-8 cells after 1 Gy irradiation. n = 3 biological repeats. Error bars = s.d. c: Time and dosage-dependent increase of MN IR treatment according to DAPI staining. Inset: micronuclei (red arrows) visualized by DAPI staining. d: γH2AX and 53BP1 staining by IF for OVCAR-8 12 h post-IR, showing unrepaired DSBs in both MN and nuclei compartments. e: Counting of γH2AX (Left vertical axis) and percentage of cells containing MN (Right vertical axis) at indicated dosages of IR irradiation. f: Induction of MN upon various genotoxic treatments. Percentage of micronucleated cells was enumerated according to DAPI staining. Apparently, OVCAR-8 is more liable to MN formation after ionizing radiation in comparison to other treatments. Cytotoxic chemicals were treated for 4 h and their concentrations are described in Methods. UV: 5 J/m2; IR: 2 Gy