Fig. 7

Effects of SPIB knock-down on resistant cells. a Conformation of partial silencing of SPIB on gene level. The relative mRNA expression of SPIB was assessed in the cells using TaqMan probe based RT-PCR and revealed a knock of gene expression with 50% compared to un-knocked control cells. The data are normalised to the S18 reference gene. b Protein levels of SPIB measured with western blot 48 h after transfection with siRNA. GAPDH was used as loading control. Representative figure of three biological replicates. c Normalised proliferation for Z138 cells grown in cytarabine containing medium, measured by incorporation of [methyl-14C]-thymidine 72 h after knock of SPIB using siRNA. Each data point represents a mean value of triplicates and error bars show SD. * = p < 0.05, using students unpaired t-test