Fig. 5

The difference between 786-O and 786-M-R cells in cell migration and proteins. a Graphs of Immunofluorescence. PAMPK, pAKT and aH3 were analysed by immunofluorescence in 786-O cells cultured with VPA 1 mM (VPA), metformin 10 mM (Met) or a combination (VPA + Met). (200× magnification; Bar, 50 μm). b 786-O and 786-M-R cells were starved for 12 h then performed wound healing assay, wound closure was calculated. *P < 0.05 vs 786-O group. (200× magnification; Bar, 100 μm). c, d Western blot analysis of cell signalling proteins and EMT makers in 786-O and 786-M-R cells. *P < 0.05 vs 786-O group