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Table 2 Breakpoints detected within the AF9 gene in BA-treated TWO4 cells

From: Bile acids at neutral and acidic pH induce apoptosis and gene cleavages in nasopharyngeal epithelial cells: implications in chromosome rearrangement

BA-treated TWO4 cells Breakpoint Remarks
0.5 mM BA, pH 7.4 245596 This breakpoint is identical with a breakpoint identified in NP69 treated with BA at pH 7.4. This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) previously reported being involved in the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]. This breakpoint is three nucleotides different from that reported in cultured normal blood cells treated with VP16 (at coordinate 245,593) [74], five nucleotides different from a breakpoint detected in H2O2-treated NP69 cells (at coordinate 245,591) and six nucleotides different from that identified in H2O2-treated HK1 cells (at coordinate 245,590) [77].
245664 This breakpoint is five nucleotides different from a breakpoint (at coordinate 245,659) identified in NP69 cells treated with H2O2 [77].
245803 This breakpoint is seven nucleotides different from a breakpoint (at coordinate 245,796) reported in NP69 cells treated with H2O2 [77].
245935  
0.5 mM BA, pH 5.8 245509 This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene leading to the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804].
245594 This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene leading to the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]. This breakpoint is one nucleotide different from that reported in cultured normal blood cells treated with VP16 (at coordinate 245,593) [74], three nucleotides different from a breakpoint detected in H2O2-treated NP69 cells (at coordinate 245,591), and four nucleotides different from that identified in H2O2-treated HK1 cells (245590) [77].
245612 This breakpoint is identical with the breakpoint previously identified in an ALL patient [GenBank:AM050804].
245729  
246044  
246116 This breakpoint is two nucleotides different from a breakpoint (at coordinate 246,114) reported in CEM cells exposed to VP16 [74], and three nucleotides different from a breakpoint (at coordinate 246,113) identified in NP69 cells treated with H2O2 [77].
  1. The breakpoints identified within the AF9 gene were mapped according to the AF9 sequence retrieved from Ensembl database [EMBL:ENSG00000171843]