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Fig. 9 | BMC Cancer

Fig. 9

From: Bile acids at neutral and acidic pH induce apoptosis and gene cleavages in nasopharyngeal epithelial cells: implications in chromosome rearrangement

Fig. 9

The positions of bile acid-induced chromosome breakages within the AF9 gene. aThe genomic map of AF9 gene from nucleotide positions 601–281,480 is illustrated [EMBL:ENSG00000171843]. Green boxes represent the two previously identified patient breakpoint cluster regions which are denoted as BCR1 and BCR2 [89]. Yellow boxes indicate the biochemically defined MAR/SARs reported in the previous study. These two MAR/SARs were denominated as SAR1 and SAR2 [89]. B: BamH I restriction sites. b Targeted region (10.2 kb) in the AF9 gene. Green and blue arrows indicate the primers used in the first (R1, AF9 236,451 R and F1, AF9 245,385 F) and second (R2, AF9 236,211 R and F2, AF9 245,507 F) rounds of nested IPCR, respectively. BamH I (B), Kpn I (K) and Nde I (N) restriction sites are shown. c Chromosome breakages detected in the present study by using IPCR. Red and green vertical lines indicate the presently identified breakpoints in BA-treated NP69 and TWO4 cells, respectively. All the chromosome breakages were mapped within BCR1 in close proximity with SAR1. Blue box represents the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene resulting in the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]

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